A TaqI RFLP in Xq26–qter detected by pX301b [DXS311]

TaqI RFLP at D21S137.

Chromosomal Localization: The 3 polymorphic bands were mapped to chromosome 22 using a somatic cell hybrid panel. The 2.1 kb constant band mapped to chromosome lq whereas all other constant bands mapped to chromosome 20. Whereas the active gene for hTOPl is located on chromosome 20ql 1.2—13.1, two pseudogenes are on chromosomes Iq23—24 and 22qll.2-13.1 (2). The sequences of hTOPl and the two ps...

A TaqI RFLP for the human arylsulfatase A gene.

TaqI RFLP polymorphism of the ovine complement component C4 gene.

Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...

A new TaqI allele detected by the CRI-R227 (D4S101) probe in Pima Indians.

No abstract available]

A third TaqI allele is detected by the probe pTD3-21 (D15S10) in southern African chromosomes.

Primer Sequences: 5' CCT CCA CAT GGA CTC ACC TG 3' (CA strand); 5' AAG GGG AGG GAA TCA GGT GT 3' (TG strand). Polymorphism: Heterozygosity was estimated at 74% by analysis of 92 chromosomes from unrelated individuals. Allele Bl B2 B3 B4 B5 B6 B7 B8 B9 B10 bp 194 192 190 188 186 184 182 180 178 176 Frequency 0.01 0.05 0.10 0.05 0.06 0.21 0.43 ND 0.01 0.08 Chromosomal Localisation: Fluorescence i...